Intermediate

Part:BBa_S00160:Design

Designed by: Heather Keller   Group: T7.2   (2007-03-05)


R0011+B0100+B0048


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 167
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was assembled via PCR amplification rather than with standard assembly in order to reduce cloning time (less assembly steps) and for ease of handling (small parts are hard to digest and purify properly).


Source

This part was made by PCR amplification of B0100 from the MG1655 genome with the Biobricks upstream cloning sites (EcoRI and XbaI) and R0011 included in the forward promoter and B0048 and the Biobricks downstream cloning sites (SpeI and PstI) included in the reverse primer.

References